HEPPS is widely used as a buffering agent in biochemical and molecular biology applications. It is particularly effective in maintaining stable pH levels in cell culture media, enzyme assays, and protein purification processes. Its buffering capacity is optimal in the pH range of 7.0 to 8.5, making it suitable for various biological systems. HEPPS is also utilized in electrophoresis and chromatography techniques to ensure consistent pH conditions, which are critical for accurate results. Additionally, it is employed in the preparation of reagents for diagnostic tests and in the study of cellular processes, where precise pH control is essential. Its low toxicity and compatibility with biological systems make it a preferred choice in life science research.