N-Cyclohexyltaurine (CHES) is widely used as a buffering agent in biochemical and molecular biology research. Its primary application is in maintaining stable pH conditions, particularly in the range of 8.6 to 10.0, making it suitable for experiments involving enzymes, proteins, and other biological molecules that require alkaline environments. CHES is often employed in electrophoresis, chromatography, and enzyme assays where precise pH control is critical. Additionally, it is used in cell culture studies and as a component in various diagnostic kits to ensure optimal conditions for biochemical reactions. Its stability and effectiveness in alkaline pH ranges make it a preferred choice in laboratories for maintaining consistency in experimental conditions.