This chemical, known as CHES, is a zwitterionic buffering agent widely used in biochemical and molecular biology research. It helps maintain a stable pH in various experimental conditions, particularly in applications requiring alkaline pH (effective range: 8.6-10.0), such as certain enzyme studies and cell culture at higher pH. It is suitable for use in electrophoresis and chromatography techniques where alkaline conditions are needed. Additionally, it is employed in the preparation of reagents for protein purification and analysis. Its stability and compatibility with biological systems make it a valuable component in diagnostic assays and pharmaceutical formulations.