Used as a buffering agent in biochemical and biological research to maintain stable pH conditions in cell culture media and enzyme studies. It is particularly effective in the pH range of 7.0 to 8.5, making it suitable for applications in molecular biology, protein purification, and electrophoresis. It is also utilized in the preparation of solutions for tissue culture and in vitro experiments due to its compatibility with biological systems and minimal interference with cellular processes. Additionally, it serves as a stabilizing agent in diagnostic assays and helps maintain the integrity of biological samples during storage and analysis.