Used as a buffering agent in biochemical and molecular biology research, it helps maintain stable pH levels in cell cultures and enzyme reactions. Commonly employed in electrophoresis and chromatography techniques to ensure optimal separation and analysis of biological molecules. Its effectiveness in physiological pH ranges makes it suitable for studies involving proteins, nucleic acids, and other biomolecules. Additionally, it is utilized in the preparation of media for cell and tissue culture, providing a stable environment for growth and experimentation.