Earle's Balanced Salt Solution (HBSS), without calcium chloride, magnesium chloride, and phenol red, is widely used in cell and tissue culture applications. It serves as a physiological buffer to maintain optimal pH and osmotic balance during cell washing, media preparation, and experimental procedures. Due to the absence of calcium and magnesium, it is particularly useful for processes requiring chelation or the prevention of cell adhesion, such as in dissociation protocols. The exclusion of phenol red makes it suitable for assays and imaging techniques where dye interference could affect results, such as fluorescence microscopy or spectrophotometry. Additionally, it is commonly employed in flow cytometry, cell viability assays, and as a diluent for reagents in various biological research applications.