Bis-Tris is widely used as a buffering agent in biochemical and molecular biology applications. Its effective pH range of 5.8 to 7.2 makes it suitable for maintaining stable pH conditions in various experimental setups, such as protein purification and electrophoresis. It is particularly favored in gel electrophoresis for its low conductivity, which minimizes heat generation and improves resolution. Additionally, Bis-Tris is used in cell culture media to maintain physiological pH levels, supporting cell growth and viability. Its compatibility with metal ions also makes it useful in studies involving metalloproteins and enzymatic reactions.