Used in biological and biochemical experiments to maintain a stable pH in the range of approximately 8.0 to 10.5. Commonly applied in enzyme assays, protein purification, and electrophoresis where pH control is critical. Its buffering capacity supports optimal conditions for enzyme activity and protein stability. Also used in studies involving nucleic acids, such as DNA and RNA extraction and hybridization, due to its compatibility with sensitive biomolecules. Suitable for cell lysis buffers and chromatography procedures.