I-BRD9 is primarily used in research settings to study and modulate the function of bromodomain-containing proteins. These proteins play a critical role in regulating gene expression by interacting with acetylated lysine residues on histone proteins. By inhibiting BRD9, researchers can investigate its role in various cellular processes, including chromatin remodeling, transcription regulation, and epigenetic signaling. This compound is particularly valuable in cancer research, as it helps to explore the potential of targeting bromodomain proteins for therapeutic purposes, especially in diseases driven by aberrant gene expression. Additionally, I-BRD9 serves as a tool compound in chemical biology to dissect the biological pathways and mechanisms involving BRD9 and related proteins.