Used as a key intermediate in the synthesis of β-lactam antibiotics, particularly in the production of carbapenem-class antibiotics. Its bicyclic structure provides a rigid framework that mimics the core of naturally occurring β-lactams, making it valuable in designing potent antimicrobial agents. The tert-butyl ester group enhances stability and facilitates further chemical modifications during multi-step syntheses. It is also employed in medicinal chemistry for developing enzyme inhibitors targeting bacterial cell wall biosynthesis.