Used as a fluorescent probe in biological imaging due to its high photostability and bright emission. It is particularly effective in tracking cellular processes and monitoring microenvironment changes such as pH and polarity. Its nitrophenyl group enhances sensitivity to electron transfer processes, making it suitable for use in fluorescence-based sensors. Commonly applied in microscopy and flow cytometry for labeling membranes and intracellular structures. Also employed in photodynamic therapy research and as a energy donor in FRET (Förster resonance energy transfer) systems.