Used as a fluorescent probe in biological imaging due to its strong photostability and bright emission in the red to near-infrared range. Its structure allows for efficient cell membrane permeability, making it suitable for live-cell imaging without requiring transfection agents. Commonly applied in tracking intracellular pH changes and monitoring reactive oxygen species in real time. Also utilized in super-resolution microscopy techniques such as STED and PALM, where precise localization of cellular structures is required. Shows low cytotoxicity, enabling long-term observation of cellular processes. Additionally, employed in photodynamic therapy research as a photosensitizer carrier due to its ability to generate singlet oxygen upon light excitation.