Nitrocellulose stripping buffer, 10X, is primarily used in molecular biology and biochemistry laboratories for the reprobing of Western blots. After an initial round of antibody detection, this buffer helps remove the primary and secondary antibodies bound to the nitrocellulose membrane without damaging the immobilized proteins. This allows researchers to reuse the same membrane for subsequent probing with different antibodies, optimizing the use of materials and reducing costs. The buffer is particularly valuable in experiments where multiple protein targets need to be analyzed from a single sample, enabling efficient and reliable detection of various antigens on the same blot. Its effectiveness in stripping antibodies while preserving protein integrity makes it a critical tool in immunoblotting workflows.