Stripping Buffer 3 is widely used in molecular biology and biochemistry laboratories for the removal of antibodies or probes from membranes after Western blotting. This allows researchers to reuse the same membrane for multiple detections, saving time and resources. The buffer effectively strips bound antibodies and probes without damaging the membrane, making it ideal for sequential probing with different antibodies. It is particularly useful in experiments where multiple target proteins need to be analyzed from a single sample, ensuring accurate and reproducible results. Its application is critical in studies involving protein expression, post-translational modifications, and signal transduction pathways.