Bis-Tris Transfer Buffer is widely used in protein electrophoresis, particularly in Western blotting procedures. It facilitates the efficient transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes. This buffer is preferred for its ability to maintain a stable pH during the transfer process, which is crucial for preserving protein integrity and ensuring accurate results. Additionally, it is compatible with a variety of protein detection methods, making it a versatile choice in molecular biology and biochemistry laboratories. Its low conductivity also minimizes heat generation, reducing the risk of protein damage during transfer.