This 10x buffer is primarily used in Western blotting to transfer proteins from polyacrylamide gels to nitrocellulose or PVDF membranes. It ensures efficient and consistent protein transfer by maintaining the appropriate pH and ionic strength. The Tris-acetate system is particularly useful for transferring larger proteins, as it provides better resolution compared to other buffer systems. It is often used in a 1x concentration after dilution with deionized water. The buffer is compatible with various detection methods, including chemiluminescence and fluorescence, making it versatile for different experimental setups. Proper use of this buffer helps achieve clear and reliable protein bands, essential for accurate analysis and interpretation of Western blot results.