Blotto-Tween in PBS is commonly used in molecular biology and biochemistry laboratories as a blocking and washing buffer. It is particularly effective in Western blotting procedures to reduce nonspecific binding of antibodies to the membrane. The solution helps improve the signal-to-noise ratio, ensuring clearer and more accurate detection of target proteins. Additionally, it is used in immunoassays and ELISA to minimize background interference, enhancing the specificity and sensitivity of the assay. Its formulation in PBS ensures compatibility with biological samples, making it a versatile choice for various protein detection techniques.