Blotto in PBS is commonly used as a blocking agent in immunoassays, such as Western blotting and ELISA. It helps reduce non-specific binding by occupying potential binding sites on the membrane or plate. This ensures that antibodies bind specifically to their target proteins, improving the accuracy and reliability of the results. Additionally, it stabilizes proteins and prevents degradation during the assay process. Its formulation in PBS (Phosphate Buffered Saline) provides a stable and compatible environment for biological samples.