Gelatin-Tween in TBS is widely used as a blocking agent in immunoassays, such as ELISA and Western blotting, to prevent non-specific binding of antibodies to the membrane or plate. The gelatin acts as a protein blocker, while Tween (a non-ionic detergent) helps reduce background noise by minimizing hydrophobic interactions. This combination ensures higher specificity and sensitivity in detecting target proteins or antigens. It is also employed in immunohistochemistry to enhance staining clarity and reduce false positives. Its stability and effectiveness make it a preferred choice in various molecular biology and diagnostic applications.