This compound is primarily used in biochemical research and bioconjugation techniques. It serves as a versatile reagent for labeling proteins, peptides, and other biomolecules due to its ability to form disulfide bonds. The pyridyldithio group allows for selective conjugation with thiol-containing molecules, while the biotin moiety enables detection or purification through streptavidin-biotin interactions. It is particularly useful in studying protein-protein interactions, cellular processes, and developing targeted drug delivery systems. Additionally, it finds applications in affinity chromatography, immunoassays, and the preparation of biotinylated probes for diagnostic purposes.