Biotin-PEG6-t-butylester is widely used in bioconjugation and biochemical research due to its unique properties. The biotin moiety allows for strong and specific binding to avidin or streptavidin, making it valuable in affinity purification, immunoassays, and labeling applications. The PEG6 spacer enhances solubility and reduces steric hindrance, improving the efficiency of biotinylation processes. The t-butyl ester group provides stability and can be selectively deprotected under mild acidic conditions, enabling controlled release or activation of the biotin molecule. This compound is particularly useful in the development of targeted drug delivery systems, diagnostic tools, and molecular probes, where precise and stable biotinylation is essential. Its versatility makes it a key reagent in proteomics, cell biology, and nanotechnology research.