Amine-PEG3-Desthiobiotin is widely used in bioconjugation and molecular biology applications. It serves as a linker molecule, enabling the attachment of desthiobiotin to various biomolecules, such as proteins, peptides, or antibodies, through its amine-reactive group. The PEG3 spacer provides flexibility and solubility, enhancing the efficiency of bioconjugation. Desthiobiotin, a biotin analog, is particularly useful in affinity purification and detection systems, as it binds to streptavidin with high affinity but can be easily displaced under mild conditions, allowing for reversible binding. This makes it valuable in techniques like pull-down assays, protein purification, and targeted drug delivery systems. Additionally, its applications extend to diagnostic assays, where it aids in the detection and isolation of specific biomolecules.