Used primarily in biochemical research, this compound serves as a versatile linker for biotinylation. It facilitates the attachment of biotin to various molecules, enabling their detection or purification through biotin-streptavidin interactions. Commonly applied in protein labeling, it helps in studying protein interactions, localization, and function. Additionally, it is utilized in immunoassays and diagnostic kits to enhance sensitivity and specificity. Its dual carboxyl groups allow for further conjugation, making it valuable in designing probes and affinity matrices for targeted biomolecule isolation.