This chemical is primarily used in bioconjugation and chemical biology research. It serves as a linker molecule, enabling the attachment of biotin to various biomolecules like proteins, peptides, or nucleic acids. The PEG3 spacer enhances solubility and reduces steric hindrance, facilitating efficient binding interactions. The amine group allows for further functionalization, making it versatile for creating biotinylated probes or affinity tags. These applications are particularly valuable in techniques such as pull-down assays, ELISA, and fluorescence labeling, where biotin-streptavidin interactions are utilized for detection or purification purposes.