Sulfo-NHS-Biotin is widely used in biochemical research for labeling proteins, peptides, and other amine-containing biomolecules. It is particularly valuable in biotinylation processes, where it enables the attachment of biotin to target molecules via primary amine groups. This biotinylation allows for the subsequent use of streptavidin or avidin-based detection systems, which are highly specific and sensitive. Applications include protein purification, immunoprecipitation, and Western blotting, where it helps in the isolation and identification of proteins. It is also utilized in cell surface labeling to study membrane proteins and their interactions. Additionally, Sulfo-NHS-Biotin is employed in the development of diagnostic assays and in the study of protein-protein interactions, making it a versatile tool in molecular biology and biochemistry research.