This compound is widely used in biochemical research for biotinylation processes. It features an extended PEG-like linker (4,7,10,13-dioxanonanoic acid chain), which provides a longer spacer arm of approximately 15 atoms between the biotin moiety and the N-hydroxysuccinimide (NHS) ester, reducing steric hindrance and improving conjugation efficiency with larger biomolecules. It serves as a key reagent for labeling proteins, peptides, and other biomolecules with biotin, enabling their detection, purification, or immobilization. The NHS ester group facilitates efficient conjugation with primary amines, ensuring stable covalent bonds. Applications include immunoassays, flow cytometry, and affinity chromatography, where biotin-streptavidin interactions are utilized for high-specificity binding. It is also employed in the development of diagnostic tools and targeted drug delivery systems.