N-Biotinyl-1,6-hexanediamine is widely used in biochemical research and bioconjugation techniques. Its primary application is as a linker molecule for attaching biotin to various biomolecules, such as proteins, peptides, or nucleic acids. This enables the use of biotin’s strong affinity for avidin or streptavidin in detection and purification processes, such as in ELISA, Western blotting, or affinity chromatography. The hexanediamine spacer provides flexibility and distance between the biotin moiety and the target molecule, ensuring optimal interaction with avidin-based systems. Additionally, it is utilized in the development of biotinylated probes for studying molecular interactions, cellular imaging, and diagnostic assays. Its stability and specificity make it a valuable tool in biotechnology and molecular biology applications.