Used as a biochemical reagent in research and diagnostic applications, particularly in studies involving biotin-binding proteins and avidin-biotin systems. It serves as a derivatizing agent for labeling biomolecules, enabling their detection and purification in assays like ELISA, immunohistochemistry, and Western blotting. Its water-soluble sodium salt form enhances compatibility with aqueous solutions, making it suitable for use in biological and enzymatic studies. Additionally, it is employed in the development of biotinylated probes for targeted drug delivery and imaging techniques.