This chemical is primarily used in biochemical research for labeling and tracking biomolecules. Its structure allows for efficient conjugation with proteins, peptides, or other biological molecules, enabling visualization and analysis in cellular and molecular studies. The propargyl group facilitates click chemistry reactions, making it a valuable tool for bioorthogonal labeling. Additionally, the PEG4 spacer enhances solubility and reduces steric hindrance, improving binding efficiency. It is commonly applied in fluorescence microscopy, flow cytometry, and other imaging techniques to study cellular processes, protein interactions, and localization in live or fixed cells.