DBCO-Biotin is widely used in bioorthogonal chemistry for labeling and detecting biomolecules. Its primary application is in click chemistry reactions, where it enables the specific and efficient conjugation of biotin to azide-containing molecules. This is particularly useful in proteomics and cellular imaging, as it allows for the precise tagging and visualization of proteins, nucleic acids, and other cellular components. The biotin tag facilitates subsequent purification or detection using streptavidin-based systems, making it a valuable tool in affinity chromatography, ELISA, and fluorescence microscopy. Additionally, DBCO-Biotin is employed in drug delivery research to target and track therapeutic agents in biological systems. Its stability and selectivity under physiological conditions make it a preferred choice for advanced bioconjugation strategies.