Used in bioconjugation and affinity labeling applications, this compound serves as a key reagent for attaching biotin to biomolecules such as proteins, peptides, or antibodies. Its primary function is to enable detection, purification, or immobilization of target molecules through strong streptavidin-biotin interactions. The amine-reactive nature allows it to form stable amide bonds with carboxyl groups when activated, making it suitable for labeling strategies in immunoassays, flow cytometry, and pull-down assays. The trifluoroacetate salt form enhances solubility and stability in common organic and aqueous-organic solvents, facilitating use in diverse biochemical workflows. Commonly employed in proteomics and molecular biology research for tagging and isolation of biomolecular complexes.