Used in bioconjugation processes to label biomolecules for detection and purification in molecular biology research. The biotin moiety enables strong binding to streptavidin or avidin, making it useful in immunoassays, ELISA, and blotting techniques. The 1,3-cyclopentanedione group acts as a reactive handle for coupling with amine-containing compounds under mild conditions. This compound is particularly valuable in developing diagnostic probes and targeted drug delivery systems where precise biomolecule tagging is required. Its stability and specificity enhance sensitivity in detection applications.