Used in bioconjugation processes to link biotin to other molecules such as proteins, peptides, or nucleic acids. The terminal amine group enables coupling to carboxyl-activated or other electrophilic groups on target molecules. The C5 spacer provides improved accessibility to streptavidin or avidin binding sites, enhancing detection sensitivity in assays like ELISA, flow cytometry, and immunohistochemistry. Commonly employed in the development of diagnostic probes and targeted drug delivery systems due to its strong affinity for streptavidin and low non-specific binding. Also utilized in immobilization strategies for biosensors and solid-phase extraction where controlled orientation of biomolecules is required.