Orfamide B is a lipopeptide biosurfactant produced by Pseudomonas bacteria, known for its dual roles in biocontrol and surfactant applications in agriculture. It exhibits potent antifungal activity against plant pathogens such as Fusarium and Pythium species, which cause root and crown rot in various crops, while also promoting plant growth and stimulating the plant immune system to enhance disease resistance. Due to its surfactant properties, Orfamide B improves the spreading, wetting, and adherence of liquids on plant surfaces, thereby increasing the efficacy of pesticides, herbicides, and fungicides through more uniform coverage. It is also utilized in irrigation systems to reduce surface tension, enabling efficient water distribution and soil penetration. Furthermore, its ability to promote biofilm formation supports bioremediation efforts by aiding in the degradation of environmental pollutants. Overall, Orfamide B contributes to sustainable agricultural practices by reducing reliance on synthetic chemicals and optimizing environmental management.