17a-Hydroxypregnenolone is primarily used in the study of steroid hormone biosynthesis. It serves as a key intermediate in the production of other steroid hormones, including cortisol, androgens, and estrogens. Researchers utilize it to understand metabolic pathways and enzyme functions involved in steroidogenesis. It is also employed in clinical studies to investigate disorders related to steroid hormone imbalances, such as congenital adrenal hyperplasia. Additionally, it is used in pharmaceutical research to develop treatments targeting hormone-related conditions.