Widely used in cell culture and microbiological media to support the growth of certain bacteria, yeast, and mammalian cells due to its role as a coenzyme in carboxylation reactions.

Commonly added to hair, skin, and nail supplements because it supports keratin production and is believed to improve strength and appearance.

Used in pharmaceutical formulations for treating biotin deficiency, which can occur in individuals with metabolic disorders, long-term parenteral nutrition, or certain genetic conditions.

Employed in biotechnology and molecular biology as a labeling agent—biotin binds strongly to streptavidin, enabling detection and purification of proteins, nucleic acids, and other biomolecules in assays like ELISA, Western blotting, and immunohistochemistry.

Utilized in animal feed to promote healthy growth, improve coat condition, and support metabolic functions in livestock and pets.