DL-threo-2-methylisocitrate is primarily used in biochemical research and metabolic studies. It serves as a substrate or intermediate in enzymatic assays to investigate the activity of enzymes like isocitrate lyase and isocitrate dehydrogenase, which are crucial in the glyoxylate cycle and the tricarboxylic acid (TCA) cycle, respectively. Researchers utilize it to study metabolic pathways, particularly in microorganisms and plants, to understand carbon metabolism and energy production. Additionally, it aids in exploring the regulation of metabolic fluxes and the role of specific enzymes in cellular processes. Its application extends to the development of inhibitors or modulators for enzymes involved in these pathways, which can have implications in areas such as antimicrobial drug development or plant biotechnology.