Widely used in western blotting as a chromogenic substrate for horseradish peroxidase (HRP), enabling detection of specific proteins through a visible precipitate. It produces a blue-purple insoluble product upon oxidation, allowing clear visualization of target bands on membranes. The ready-to-use formulation simplifies the detection process by eliminating the need for solvent preparation, reducing handling steps and improving reproducibility. Suitable for use in non-radioactive detection systems where sensitivity and clarity are critical. Commonly applied in immunoblotting and immunochemistry workflows in research and diagnostic settings.