Used as a biological stain in cell viability assays, particularly in the detection of dehydrogenase enzyme activity in living cells. It is commonly applied in cytotoxicity and proliferation studies, where metabolically active cells reduce the compound to form a red-colored formazan product. This color change allows for easy quantification of cell health and is widely utilized in in vitro testing for pharmaceuticals, toxicology research, and cancer studies. Its fluorinated structure enhances membrane permeability and stability, improving sensitivity in detection.