This compound is widely used in bioconjugation chemistry, particularly for labeling proteins and other biomolecules. Its azido group allows for efficient click chemistry reactions, such as the copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling the attachment of various functional groups or probes to biomolecules. The succinimidyl ester moiety reacts selectively with primary amines, making it ideal for modifying lysine residues in proteins. This chemical is especially valuable in proteomics and bioimaging, where it helps in tagging proteins for detection, tracking, or purification purposes. Additionally, it is employed in the development of antibody-drug conjugates (ADCs) and other therapeutic bioconjugates, enhancing targeted drug delivery systems. Its nitro group can also serve as a spectroscopic tag or participate in further chemical modifications, expanding its utility in research and industrial applications.