This chemical is primarily used as a reactive dye intermediate in biochemical research, particularly for labeling proteins and peptides. It serves as a crosslinking agent, enabling the covalent attachment of fluorescent or chromophoric groups to biomolecules. This facilitates the study of protein interactions, localization, and dynamics in various experimental setups. Additionally, it is employed in immunohistochemistry and fluorescence microscopy for visualizing cellular components with high specificity. Its ester group reacts efficiently with primary amines, making it a valuable tool for modifying and tracking biomolecules in complex biological systems.