Used as a key reagent in bioconjugation processes, it facilitates the coupling of biomolecules such as proteins, peptides, and antibodies with other molecules or surfaces. Commonly employed in the preparation of affinity chromatography resins, it helps immobilize ligands onto solid supports for protein purification. It is also utilized in the synthesis of active esters for peptide coupling reactions, enhancing the efficiency of amide bond formation. In diagnostic applications, it aids in the labeling of antibodies and proteins with fluorescent dyes or other markers for detection and imaging. Additionally, it plays a role in crosslinking reactions for studying protein-protein interactions and in the development of drug delivery systems.