Used extensively as a fluorescent labeling reagent in biochemical and cellular studies. It is particularly effective for labeling proteins, peptides, and antibodies due to its reactive N-succinimidyl ester group, which forms stable amide bonds with primary amines. The compound's coumarin derivative provides strong fluorescence, making it ideal for tracking and imaging applications. It is commonly employed in fluorescence microscopy, flow cytometry, and immunoassays to visualize and analyze biological molecules and cellular processes. Its high sensitivity and specificity make it a valuable tool in research focused on protein interactions, localization, and dynamics.