Used in biochemical research, this compound is primarily employed as a linker molecule in bioconjugation techniques. It facilitates the attachment of biotin to various biomolecules, enabling their detection and isolation in assays such as ELISA, Western blotting, and affinity chromatography. Its hexanediamine spacer provides flexibility and optimal spacing, enhancing binding efficiency in molecular interactions. Additionally, it is utilized in the development of diagnostic tools and targeted drug delivery systems, leveraging biotin’s strong affinity for avidin or streptavidin.