This chemical is primarily used in bioconjugation and biochemical research, where it serves as a linker molecule for attaching biotin to proteins, peptides, or other biomolecules. Its polyethylene glycol (PEG) spacer arm enhances solubility and reduces steric hindrance, making it ideal for applications in affinity chromatography, immunoassays, and protein labeling. The biotin moiety allows for strong binding to avidin or streptavidin, enabling precise detection, purification, and immobilization of target molecules in various experimental setups. It is particularly valuable in studies involving protein-protein interactions, cell surface labeling, and diagnostic assays.