This compound is primarily utilized in bioorthogonal chemistry, particularly in the field of bioconjugation. It serves as a reactive handle for the inverse electron-demand Diels-Alder (IEDDA) reaction, enabling the selective labeling and modification of biomolecules such as proteins, peptides, and nucleic acids. Its tetrazine moiety reacts rapidly and specifically with trans-cyclooctene (TCO) or other dienophiles, making it valuable for live-cell imaging, drug delivery systems, and the development of targeted therapeutics. Additionally, it is employed in the synthesis of advanced materials and probes for studying biological processes in real-time with minimal interference.