1-(4-Isothiocyanatobenzyl)ethylenediamine-N,N,N′,N′-tetraacetic acid is a bifunctional chelating agent used in biochemical and cellular assays. It features an isothiocyanate group that enables covalent labeling of amine groups on proteins and other biomolecules, facilitating conjugation for targeted detection. The ethylenediaminetetraacetic acid (EDTA) moiety provides strong metal-chelating properties, useful for stabilizing divalent cations or incorporating metal ions in labeling strategies. This compound is applied in immunofluorescence, flow cytometry, confocal microscopy, and immunoassays to track and detect specific cellular targets, enhancing assay sensitivity and specificity.