Used in bioconjugation and molecular labeling due to its biotin moiety, enabling strong interaction with streptavidin or avidin in assays like ELISA, flow cytometry, and immunohistochemistry. Commonly employed in probe development for protein detection, purification, and immobilization on solid supports. Also utilized in peptide-based research to track cellular uptake or study receptor binding when linked to active compounds. Its amide group enhances stability in biological systems, making it suitable for in vitro and in vivo applications.