Used as a coupling agent in peptide synthesis, particularly in solid-phase peptide synthesis where it helps in the efficient formation of amide bonds. It activates carboxylic acids, enabling smooth coupling with amines while minimizing racemization. Its structure allows for good solubility in polar solvents, making it suitable for use in automated synthesis and pharmaceutical development. Also employed in bioconjugation processes for labeling biomolecules and in the preparation of bioactive compounds where high coupling efficiency is required.