Used primarily as a photoaffinity labeling agent in biochemical and pharmacological research. Its key application lies in covalently tagging target proteins or receptors upon UV irradiation, allowing scientists to study drug-receptor interactions, enzyme active sites, and protein-ligand binding. The diazirine ring generates highly reactive carbenes when exposed to light, which insert nonspecifically into nearby C–H, N–H, or O–H bonds, forming stable covalent links. The bromoethyl group can serve as a handle for further functionalization or conjugation with biomolecules. This compound is especially valuable in mapping binding sites and identifying unknown protein targets in complex biological systems.